Megazyme 内切木聚糖酶测定试剂盒（xylx6法）

Content: (K-XylX6-1V)

100 assays (manual) / 200 assays (auto-analyser)

(K-XylX6-2V)

200 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: endo-1,4-β-Xylanase

Assay Format: Spectrophotometer, Auto-analyser

Detection Method: Absorbance

Wavelength (nm): 400,

405  

Signal Response: Increase

Limit of Detection: 5.3 x 10-4 U/mL

Reproducibility (%): ~ 3%

Total Assay Time: 10 min

Application examples: Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis.

Method recognition: Novel method

Advantages

Very cost effective 

All reagents stable for > 4 years 

Completely specific for endo-1,4-β-xylanase 

Generally applicable and highly sensitive 

Simple format. Well suited to automation 

Excellent reproducibility 

Standard included

The K-XYLX6-1V pack size has been discontinued (read more)

The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Need to measure other enzyme activities? View our complete list of assay kits for measurement of enzyme activity.

Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file under the Documents tab.

含量：（K-XylX6-1V）

100次测定（手动）/200次测定（自动分析仪）

（K-XylX6-2V）

200次测定（手动）/400次测定（自动分析仪）

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：内-1,4-β-木聚糖酶

化验格式：分光光度计、自动分析仪

检测方法：吸光度

波长（nm）:400，

405

信号响应：增加

检测限：5.3 x 10-4 U/mL

再现性（%）：~3%

总测定时间：10分钟

应用实例：发酵液，工业酶制剂，动物饲料，生物燃料研究，大麦芽分析。

方法识别：一种新颖的方法

优点

极具成本效益

所有试剂稳定期>4年

对内切-1,4-β-木聚糖酶完全特异

一般适用且高度敏感

简单的格式。非常适合自动化

出色的再现性

包含标准

K-XYLX6-1V包装尺寸已停产（阅读更多）

用于测量内切木聚糖酶（内切-1,4-β-木聚糖酶）的XylX6分析试剂包含两种成分；1） 4,6-O-（3-酮丁内酯）-4-硝基苯基-β-D-45葡糖基-木五糖苷和2）β-木糖苷酶。酮封闭基团阻止β-木糖苷酶或其他外显糖苷酶对XylX6底物的任何水解作用。与内切木聚糖酶孵育可产生非封闭的色度寡糖，该寡糖可被辅助的β-木糖苷酶快速水解。因此，4-硝基苯酚的形成速率与木聚糖酶对XylX6的水解直接相关。终止反应并在加入Tris缓冲溶液（pH＝10.0）时形成酚酸酯颜色。

需要测量其他酶的活性吗？查看我们的酶活性测定试剂盒的完整列表。

请注意，在“文件”选项卡下的支持信息文件中提供了使用XylX6测定获得的吸光度与天然底物（小麦阿拉伯木聚糖和山毛榉木聚糖）上的内切木聚糖酶活性相关的标准曲线。