Cellafluor试剂
英文名：Cellafluor Reagent
货号：MR-CELLFLR
规格：100 assays (manual) / 1000 assays (auto-analyser)
报价：￥3000.00
商品描述
Cellafluor is a fluorimetric reagent that can be used for the assay of cellulase (endo-1,4-β-glucanase). The reagent consists of 2 components:
1) 4,6-O-Benzylidene-4-methylumbelliferyl-β-cellotrioside
2) β-glucosidase (Thermotoga maritima)
Upon incubation with a cellulase (endo-1,4-β-glucanase) enzyme, the functionalised trisaccharide is cleaved and the ancillary β-glucosidase can act to hydrolyse the fragment containing the 4-methylumbelliferyl group. The rate of formation of 4-methylumbelliferyl is directly proportional to the cellulase (endo-1,4-β-glucanase) activity. The assay reaction is terminated and full fluorescence obtained from the fluorophore by the addition of Tris buffer.
文档
R-CELLFLR_DATA.pdf: 