ROCHE 过氧化物酶 (POD) I 级，来自辣根

过氧化物酶 (POD) I 级，来自辣根

酶委员会编号：1.11.1.7 (BRENDA, IUBMB)

 

特性

质量等级：100

形式：冻干（无盐）

比活性：≥250 units/mg蛋白质（25°C，愈创木酚和H2O2为底物。）

250 单位/毫克

包装：每包 25,000 U

制造商/商品名：罗氏

最适pH：6.0-6.5

发货地：湿冰

储存温度：2-8°C

 

描述

一般说明

供体：过氧化氢氧化还原酶

过氧化物酶 (POD) (E.C. 1.11.1.7) 在大多数水果和蔬菜中广泛表达。

应用

过氧化物酶 (POD) 已在 AUR（Amplex UltraRed 测定）中用于测量由分解的蛞蝓细胞产生的细胞外 H2O2。

电子供体的氧化（例如，双键的环氧化）。过氧化物酶可以通过其氨基以及其碳水化合物部分与其他蛋白质偶联。重新配制的溶液可直接用于偶联，无需事先透析。它已用于配体印迹。

生化/生理作用

过氧化物酶 (POD) 参与大量有机化合物的脱氢，包括苯酚和芳香胺、对苯二酚和对苯二酚胺，主要是联苯胺衍生物。

质量

污染物：<0.001% ATPase 和酸性磷酸酶，<0.7% 过氧化氢酶

纯度数：约 3.0 (A403/A275)

准备说明

活化剂：氨、吡啶、咪唑，pH>7；洗涤剂。

工作溶液：PBS（磷酸盐缓冲盐水）或水（起始浓度 20 mg/ml）。

重组

溶液（10 mg/ml 水溶液）：澄清，红棕色； pH 值：6.0 至 7.0。

其他注意事项

仅用于生命科学研究。不用于诊断过程。 

Guò yǎnghuà wù méi (POD) I jí, láizì là gēn

 

Peroxidase (POD)grade I, from horseradish

Enzyme Commission number:1.11.1.7 (BRENDA, IUBMB)

 

PROPERTIES

Quality Level:100

form:lyophilized (salt-free)

specific activity:≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.)

250 U/mg

packaging:pkg of 25,000 U

manufacturer/tradename:Roche

optimum pH:6.0-6.5

shipped in:wet ice

storage temp.:2-8°C

 

DESCRIPTION

General description

Donor:hydrogen-peroxide oxidoreductase

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

Application

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H2O2 produced from disaggregated slug cells.

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Biochem/physiol Actions

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

Quality

Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase

Purity number: approximately 3.0 (A403/A275)

Preparation Note

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.

Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

Reconstitution

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.