黄曲霉素 b&g 免疫亲和柱

 

其功能机制基于免疫亲和层析原理，具有增加的流速和适合的低洗脱体积以节省处理时间。 柱子中的材料涂有针对黄曲霉素 B 和 G 的抗体。样品提取物上样到柱子上。 当样品提取物上样时，黄曲霉素 B 和 G 保留在色谱柱中，而基质成分通过色谱柱。 在洗涤步骤之后，黄曲霉素 B 和 G 可以用甲醇从柱子上定量洗脱，然后用 HPLC 分析。

 

aflatoxin b&g immunoaffinity column

 

The functional mechanism is based on the principle of immunoaffinity chromatography with increased flow rates and an adapted, low elution volume to save processing time. The material in the column is coated with antibodies directed against aflatoxin B and G. The sample extract is loaded onto the column. When the sample extract is loaded, the aflatoxins B and G are retained in the column while the matrix components pass through the column. After a washing step, the aflatoxins B and G can be quantitatively eluted from the column by methanol and subsequently analysed by HPLC.