商品描述
Alarey Modified NTP In Vitro Transcription: INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit
For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing,in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40 µg RNA from 1 µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped.
改良的 NTP 体外转染:INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA 转染试剂盒
用于使用 T7 RNA 聚合酶和抗反向帽类似物 (ARCA) 对含有 5-甲基胞嘧啶-假尿苷的体外转染 RNA 进行共转染控制。 5mC-Ψ-mRNAs 在哺乳动物细胞中诱导较低的先天免疫反应。一个反应在 3 小时内从 1 µg DNA 产生 40 µg RNA。反应产物 80% 正确加盖,20% 未加盖。