胰蛋白酶，重组，GMP级

Trypsin is a widely used serine protease, typically isolated from pancreas of different animals, that specifically cleaves at the C-terminus of arginine and lysine within a peptide chain. Roche has chemically synthesized a gene encoding for the amino acid sequence of Trypsin and has transformed the gene into the expression host Pichia pastoris, which expresses the recombinant Trypsin as active protease with identical properties compared to the native Trypsin.

The product is manufactured according to DIN EN ISO 13485. No animal-derived products are used in the fermentation, purification and final formulation. The production process is validated resulting in a very high lot-to-lot consistency.

Application

Use the animal component-free and DIN EN ISO 13485-manufactured Trypsin, recombinant, as critical raw material for the production of active pharmaceutical ingredients (API), i.e., insulin, vaccines and for cell dissociation.

Properties

CAS Number: 9002-07-7

Molecular weight: 23.5 kD

pH optimum: 8.0

Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain

Specifications

Appearance: Clear, colorless to slightly yellowish solution

Storage buffer: HCl, 10 mmol/L; CaCl2, 20 mmol/L

pH value: 2±0.5

Activity (with chromozym TRY): ≥10,800 U/mL

Total activity (with chromozym TRY): 3.5 MU ±10%

Specific activity: ≥180 U/mg

Protein: 70±10 mg/mL

Purity (RP-HPLC): ≤20% α-trypsin, ≥70% β-trypsin, corresponds to master lot

Bioburden: ≤100 CFU/mL

Stability: At -15 to -25°C within specification range for 36 months.

Regulatory Disclaimer

For further processing only.