Megazyme 过氧化氢检测试剂盒(Megaplex Red) K-MRH2O2

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.

 

Display all assay test kits available.

 

Fluorometric/UV method for the determination of H2O2 content in a variety of samples.

 

Content: 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Hydrogen Peroxide

Assay Format: Spectrophotometer, Microplate, Auto-analyser

Detection Method: Absorbance, Fluorescence

Wavelength (nm): (Fluorescence) 530-560 (ex), ~ 590 (em)

(Absorbance) 570

Signal Response: Increase

Linear Range: (Fluorescence) 0.0034-0.17 µg of H2O2 per assay (0.1-5 µM in-assay)

(Absorbance) 0.034-0.68 µg of H2O2 per assay (1-20 µM in-assay)

Limit of Detection: (Fluorescence) 0.0034 ug/mL

(Absorbance) 0.25 ug/mL

Reproducibility (%): (Fluorescence) ~ 8%

(Absorbance) ~ 8%

Reaction Time (min): (Fluorescence) 30 min

(Absorbance) 30 min

Application examples: Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).

 

Advantages

Simple format

Very competitive price (cost per test)

Ability to run in fluorescence or absorbance mode

User friendly - Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.

Mega-Calc™ software tool is available from our website for hassle-free raw data processing

Suitable for manual, microplate and auto-analyser formats

All reagents stable for > 2 years

 

过氧化氢测定试剂盒为过氧化氢的测量提供了一种简单可靠的方法。该试剂盒利用高灵敏度的Megaplex Red探针，在辣根过氧化物酶（HRP）存在的情况下，可以测量样品中的H2O2。在探针被HRP氧化后，形成有色产物（间苯二酚），其可以在荧光模式下使用530-560nm范围内的激发和在~590nm处的发射或在570nm处的色度测量。Megaplex红的激发和发射最大值分别为571nm和585nm。

显示所有可用的化验检测试剂盒。

荧光/紫外法测定各种样品中H2O2含量。

含量：1000次测定（微孔板）/100次测定（手动）/400次测定（自动分析仪）

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：过氧化氢

测定形式：分光光度计、微孔板、自动分析仪

检测方法：吸光度、荧光

波长（nm）：（荧光）530-560（ex），~590（em）

（吸光度）570

信号响应：增加

线性范围：（荧光）每次测定0.0034-0.17µg H2O2（测定中为0.1-5µM）

（吸光度）每次测定0.034-0.68µg H2O2（测定中为1-20µM）

检测限：（荧光）0.0034 ug/mL

（吸光度）0.25 ug/mL

再现性（%）：（荧光）~8%

（吸光度）~8%

反应时间（分钟）：（荧光）30分钟

（吸光度）30分钟

应用示例：食品和饮料样品，如牛奶或奶制品、葡萄酒、果汁、纸张（和纸板）、废物处理样品和其他材料（如生物培养物等）。

优点

简单格式

极具竞争力的价格（每次测试的成本）

在荧光或吸收模式下运行的能力

用户友好-提供了详细的协议，用于创建校准曲线和计算荧光模式下的浓度。单点标准，在吸光度模式下进行更快、更简单的分析。

Mega Calc™我们的网站上提供了软件工具，可以轻松处理原始数据

适用于手动、微板和自动分析仪格式