内切纤维素酶检测试剂盒(CELLG5方法)

Content: (K-CellG5-4V)

120 / 240 assays (manual) / 480 assays (auto-analyser) or 

(K-CellG5-2V)

60 / 120 assays (manual) / 240 assays (auto-analyser)

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: endo-Cellulase

Assay Format: Spectrophotometer, Auto-analyser

Detection Method: Absorbance

Wavelength (nm): 400

Signal Response: Increase

Limit of Detection: 1.2 x 10-3 U/mL

Reproducibility (%): ~ 3%

Total Assay Time: 10 min

Application examples: Fermentation broths, industrial enzyme preparations and biofuels research.

Method recognition: Novel method

Advantages

Very cost effective 

All reagents stable for > 4 years 

Completely specific for cellulase (endo-1,4-glucanase) 

Generally applicable and highly sensitive 

Simple format. Well suited to automation 

Standard included

The K-CellG5-2V pack size has been discontinued (read more).

Cellulase Activity Assay Kit.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

内容：（K-CellG5-4V）

120/240测定（手动）/480测定（自动分析仪）或

（K-CellG5-2V）

60/120测定（手动）/240测定（自动分析仪）

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：内切酶

化验格式：分光光度计、自动分析仪

检测方法：吸光度

波长（nm）：400

信号响应：增加

检测限：1.2 x 10-3 U/mL

再现性（%）：~3%

总测定时间：10分钟

应用实例：发酵液、工业酶制剂和生物燃料研究。

方法识别：一种新颖的方法

优点

极具成本效益

所有试剂稳定期>4年

对纤维素酶（内切-1,4-葡聚糖酶）完全特异

一般适用且高度敏感

简单的格式。非常适合自动化

包含标准

K-CellG5-2V包装尺寸已停产（阅读更多）。

纤维素酶活性测定试剂盒。

用于测定内切纤维素酶（内切-1,4-β-葡聚糖酶）的CellG5测定试剂包含两种成分；1） 4,6-O-（3-酮丁内酯）-4-硝基苯基-β-D-纤维五糖苷（BPNPG5）和2）耐热β-葡萄糖苷酶。酮封闭基团阻止β-葡萄糖苷酶对BPNPG5的任何水解作用。与内切纤维素酶孵育可产生非封闭的色度低聚糖，该低聚糖可被辅助的β-葡萄糖苷酶快速水解。因此，4-硝基苯酚的形成速率与内切纤维素酶对BPNPG5的水解直接相关。终止反应并在加入Tris缓冲溶液（pH 9.0）时形成酚酸酯颜色。

CellG5测定法代表着纤维素酶测量方法的巨大进步，纤维素酶测量方法传统上依赖于基质，如CM纤维素、Avicel、纤维低聚糖、滤纸或染色多糖，包括CMC刚果红或天青纤维素。